1. Principles,  Practices and Hardware

George Church (Harvard), Megan Palmer (Stanford), David Kong (MIT), Jean-michel Molenaar (Tufts)

 

Risk/Safety Level: What is the Safety Level of Your Lab (e.g. BSL1, BSL2, other)? Do you have different spaces with different safety levels? If so, describe which activities are done in different spaces. Include a picture of your lab.

 

The safety level of the UTEC lab is BSL2.

Room 01: Bioengineering
In this space there are two work tables, a sink and an area for cell culture.
The work table 01 contains two microscopes, a computer, a centrifuge with capacity for 04 people.
The work table 02 contains a tissue printer, an electrophoresis chamber, a thermal cycler and an analytical balance with a capacity for 04 people.
The equipment you have is a laminar luxury cabin, a freezer, an autoclave, some storage shelves.
Room 02: Engineering
In this space there are 04 work tables with capacity for 04 people. A table has a 3D printer and a computer. Another table has an open centrifuge.

 

A. Standard Microbiological Practices

1. The member of laboratory must enforce the institutional policies that control access to the laboratory with a training, the lab have a biometric recognition to access to rooms and cameras.
2. Persons must wash their hands before and after of the laboratory.
3. Not be permitted in laboratory areas eat, drink, smoke, and storing food in refrigerator of lab and heat food in the microwave.
5. The room have red bags for disposal of polluting material and organic waste and additionally, red puncture-resistant containers to syringes, scalpels, pipettes, and broken glassware.

7. Decontaminate work surfaces after completion of work with appropriate disinfectant to work with pathogens or contaminant material.

 

B. Special Practices

none

C. Safety Equipment (Primary Barriers and Personal Protective Equipment)

 

1. Protective laboratory gowns are obligation to work in laboratory.
   The gloves are used to protect hands to hazardous material, this gloves should will be change when is necessary, no reuse, no wash.

 

D. Laboratory Facilities (Secondary Barriers)

1. Laboratories have doors for biometric access control
2. The laboratory has a sink to wash your hands.
3. The laboratory is designed for easy cleaning, the divisions are tempered glass and air conditioning.
4. Laboratory furniture is capable of supporting machine loads. The spaces between banks, cabinets and equipment are accessible for cleaning.
5. The tables and chairs are waterproof and resistant to heat, organic solvents, acids, alkalis and other chemicals and can be easily decontaminated with an appropriate disinfectant.
6. The windows of the laboratories that open to the outside are sealed.
7. An environment for cell culture is isolated by glass and in it is a laminar flow cabinet, incubator and biosecurity materials.

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Design, development and execution of a turbidity sensor.

 

Turbidity is the most difficult property of water to analyse. Since turbidity is the optical property of water; it has quite many ways to measure the amount in water.

Turbidity is the total amount of particles in the water, which makes waters visibility worse, as a result of which turbidity is considered as the optical property of water. Hence, for determining turbidity, optical devices are used. For example, sending the light from one element and receiving by the other. According to the amount of light received or scattered turbidity is measured. When the suspended solid amount increases, the water’s turbidity level (and cloudiness or haziness) increases. Turbidity sensors are used in river and stream gazing, effluent measurements in ponds, wastewater and control instrumentation for settling ponds, sediment research, and various laboratory measurements.

 

The arduino turbidity sensor detects water quality by measuring the levels of turbidity. It uses light to detect suspended particles in water by measuring the light transmittance and scattering rate, which changes with the amount of total suspended solids (TSS) in water. As the TTS increases, the liquid turbidity level increases.

Turbidity sensors are used to measure water quality in rivers and streams, wastewater and effluent measurements, control instrumentation for settling ponds, sediment transport research and laboratory measurements. 

This liquid sensor provides analog and digital signal output modes. For this project we use digital system.

 

Specification

Operating Voltage: 5V DC

Operating Current: 40mA (MAX)

Response Time  : <500ms

Insulation Resistance: 100M (Min)

Output Method:

Digital Output: High/Low level signal (you can adjust the threshold value by adjusting the potentiometer)

Operating Temperature: 5℃~90℃

Storage Temperature: -10℃~90℃

Weight: 30g

Adapter Dimensions: 38mm*28mm*10mm

 

Connection Diagram

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Digital output mode

 

int ledPin = 13;                // Connect an LED on pin 13
int sensor_in = 2;                 // Connect turbidity sensor to Digital Pin 2

void setup(){
 pinMode(ledPin, OUTPUT);      // Set ledPin to output mode
 pinMode(sensor_in, INPUT);       //Set the turbidity sensor pin to input mode
}

void loop(){   

 

if(digitalRead(sensor_in)==LOW){       //read sensor signal
       digitalWrite(ledPin, HIGH);   // if sensor is LOW, then turn on
    }else{
       digitalWrite(ledPin, LOW);    // if sensor is HIGH, then turn off the led
    }
}

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The low cost turbidimeter could be very useful in the Amazon, where water quantities can affect malnutrition and diseases associated with water contamination.

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