(1) Culture bacteria from a fermented food (yogurt, cheese, kombucha, kraut, kimchi, etc.).

 

We prepared LB plates agar, We roll a clean cotton swab in the mouth.

We incubate at room temperature for 2 days at 37 C.

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(2) Co-culture experiments.

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We use three types of material, one of PLA printed in 3D, one glass and one plastic falcon 50 ml.

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(3) 3D print a 14 mL culture tube in at least one material. Culture a bacterial strain of your choice (potentially from 1 or 2 or with E. coli as a positive control) in this tube and compare the growth rate (optical density) over time versus a polystyrene control tube.

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The OD was measured in each tube:
The colony shape and color indicate that the growth was for an Escherichia coli were cultured for 24 hours in a shaking incubator at 37 ° C in a standard culture tube of 14ml and two tubes printed in 3D (PLA), of the http model were used : //metafluidics.com/devices/14-ml-culture-tube/ 500ul of culture was inoculated in each tube with 9 ml of LB 1x.
The OD measurements for these diluted cultures were 0.87 (polypropylene), 0.72(PLA) and 0 for the LB 1x cuvette.

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We need more replicas to verify the data measurements, it was only done using one type of material for each tube in OD.

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(3b) Fabricate your device, or at least one component of your device. Document the following aspects of fabrication and function in your class page:

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What features of your organ are you attempting to emulate?

I am trying to emulate the glandular organ located in the chest (breast), to study the effect of multiple drugs and effect on cell migration.

How is your device intended to function?

The device will consist of a microfluidic system connected to a pump that will drive the liquid of cell culture medium and drugs. Then it will pass through a cavity that has a hydrogel layer as its surface, the hydrogel will be the base for the breast cells and will feed the cells by capillary action of the liquid.

Were you able to fabricate your device?

Yes, I was able to manufacture a fraction of the device, however they were not tested using the cancer breast cell lines, we still do not have a -80C freezer to store the cells and multiply them.

Which components?

The microfluidics and the hydrogel were manufactured, however the pump and the output system are still in process.

Which parts 'worked' and which ones didn't?

The microfluidics for transport of liquids if it works, the growth of cell lines has not yet been tested. I plan to test using bacterial culture to simulate the transfer of culture medium to the cells.

What will you aim to improve for your next iteration of design + build?

I will add components from external channels to transport fluid in a controlled and automatic way with a pump, perform the microbial growth before culturing mammary cells to test the system. I will use antibiotics (AMK) with E. coli specific for AMK resistance and another group of non-resistant E. coli to determine the control of growth and the necessary flow. Use dyes vital for bacteria to determine how long it takes to transfuse into the hydrogel and its durability. The use of these hydrogel systems allows us to characterize the way of studying, therefore it is possible to conduct a duct with the cells, which would simulate more the study of the breast. To determine cell migration, the hydrogel is an excellent compound, but I must analyze it with the mammary cells.

 

Sistema de evaluacion de celulas cancerigenas de mama​

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(Design with Beno Juarez)

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Recursos:

Shin2012. Microfluidic assay for simultaneous culture of multiple cell types
on surfaces or within hydrogels

Caballero2017. Organ-on-chip models of cancer metastasis for future personalized medicine: from chip to the patient

McDonald2002. Poly(dimethylsiloxane) as a Material for Fabricating Microfluidic Devices

Sun 2016. Cell‐on‐Hydrogel Platform Made of Agar and Alginate for Rapid,
Low‐cost, Multidimensional Test of Antimicrobial Susceptibility

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Design:

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The design were make in Rhinoceros CAD and print in Trotec Speedy 300

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